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Expression of IL-13, TNFα, and muc5AC. Lung mRNA levels of TNFα (A), IL-13 (C), IL-5 (E), and muc5AC (F) were measured by qRT-PCR. Lung protein levels of TNFα (B) and IL-13 (D) were measured by Bioplex ELISA. N = 5, †different from respective sham-infected mice, P ≤ 0.05; *different from elastase/LPS group, P ≤ 0.05; 2-way ANOVA.
