Fig. 2.

Expression of green fluorescence protein (GFP)-tagged Prdx6 in lysosome-related compartments of MLE-12 and A549 lung epithelial cells. Left panels: GFP full-length Prdx6 fusion construct (FL) and GFP control (vector) expressed in transfected MLE-12 (A) and A549 (B) cells. Middle panels: lamellar bodies (A), stained with Nile Red, and lysosomes (B), stained with LysoTracker Red. Right panels: colocalization, detected by yellow color, in the merged image. Bottom panels in A show a magnified image of the cell in the boxed field. C: colocalization of full-length GFP-tagged Prdx6 (green) with lysosome-related organelles stained with LAMP-1 (red) in MLE-12 cells. GFP:FL, GFP tag on the NH₂ terminus of Prdx6; FL:GFP, GFP tag on the COOH terminus of Prdx6.