Table 1.

Pairs of oligonucleotides used to generate GFP-tagged Prdx6 deletion and point mutants by PCR

Mutant	PCR Primers	Template Plasmid
	Prdx6 deletion mutants
1–70 aa	[Phos]-5′-TAAAAGCTTCGAATTCTGCAGTCGACGGTACCGCGG-3′	FL
	[Phos]-5′-AGCAATCAACTTAACATTCCTCTTGGCAAACTCTGG-3′
1–40 aa	[Phos]-5′-TAAAAGCTTCGAATTCTGCAGTCGACGGTACCGCGG-3′	FL
	[Phos]-5′-TGGGTGGGAAAAGAGAATGCCCCATGAATCTCCTAG-3′
1–30 aa	[Phos]-5′-TAAAAGCTTCGAATTCTGCAGTCGACGGTACCGCGG-3′	FL
	[Phos]-5′-TCCTAGGAAATCGTGGAAGCGGATGTGGCCGATGGT-3′
31–224 aa	[Phos]-5′-GATTCATGGGGCATTCTCTTTTCCCACCCACGGGAC-3′	FL
	[Phos]-5′-CATGGCTCGAGATCTGAGTCCGGACTTGTATAGTTC-3′
1–70 aa	[Phos]-5′-CGGGACTTTACCCCAGTGTGCACCACAGAACTTGGC-3′	FL
Δ(31–39) aa	[Phos]-5′-TCCTAGGAAATCGTGGAAGCGGATGTGGCCGATGGT-3′
31–40 aa	[Phos]-5′-TAAAAGCTTCGAATTCTGCAGTCGACGGTACCGCGG-3′	31–224 aa
	[Phos]-5′-TGGGTGGGAAAAGAGAATGCCCCATGAATCCATGGC-3′
31–34 aa	[Phos]-5′-GCCCCATGAATCCATGGCTCGAGATCTGAGTCCGGA-3′	31–40 aa
	[Phos]-5′-TGGGTGGGAAAAGAGAATGCCCCATGAATCCATGGC-3′
	Prdx6 point mutants
H26A	5′-CGGCCACATCCGCTTCGCCGATTTCCTAGGAGATTC-3′	FL
	5′-GAATCTCCTAGGAAATCGGCGAAGCGGATGTGGCCG-3′
S32A	5′-CACGATTTCCTAGGAGATGCATGGGGCATTCTCTTTTCC-3′	FL
	5′-GGAAAAGAGAATGCCCCATGCATCTCCTAGGAAATCGTG-3′
G30L, G34L	5′-CGCTTCCACGATTTCCTACTAGATTCATGGCTCATTCTCTTTTCCCACCCA-3′	FL
	5′-GGGTGGGAAAAGAGAATGAGCCATGAATCTAGTAGGAAATCGTGGAAGCG-3′
31–40 aa, S32A	5′-AGATCTCGAGCCATGGATGCATGGGGCATTCTCTTTTCC-3′	31–40 aa
	5′-GGAAAAGAGAATGCCCCATGCATCCATGGCTCGAGATCT-3′
31–40 aa, S38L	5′-TCATGGGGCATTCTCTTTCTGCACCCATAAAAGCTTCGA-3′	31–40 aa
	5′-TCGAAGCTTTTATGGGTGCAGAAAGAGAATGCCCCATGA-3′
31–40 aa, S38G	5′-TCATGGGGCATTCTCTTTGGCCACCCATAAAAGCTTCGA-3′	31–40 aa
	5′-TCGAAGCTTTTATGGGTGGCCAAAGAGAATGCCCCATGA-3′

GFP, green fluorescent protein; Prdx6, peroxiredoxin 6; FL, full-length Prdx6.