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2-Cyano-3,12-dioxoolean-1,9-dien-28-oic acid (CDDO) and 15d-PGJ2 but not rosiglitazone induce heme oxygenase-1 (HO-1). Primary human lung fibroblasts were treated with the indicated concentrations of CDDO (A) or 15d-PGJ2 (B) or rosiglitazone. After 24 h, cells were harvested, and HO-1 expression was determined by Western blot. GAPDH was used as a loading control. C: cell lysates were also analyzed for expression of HO-2.
