Fig. 12.

A: CLC mRNA expression in alveolar epithelial cells. RT-PCR of the CLC1 and CLC6 isoforms demonstrates the absence of CLC1 in either TI or TII cells. CLC6, however, is present in both TI and TII cells (predicted PCR product size based on primers is 351 bp for CLC1 and 424 bp for CLC6). Our gel reveals an additional band at ∼520 in the TII cell lane for CLC6, which may represent an isoform of CLC6 derived from alternative splicing. B: CLC protein expression in alveolar epithelial cells. Western blots of TI and TII cell lysates reveal CLC5 and CLC2 are detectable in TI, but not TII, cells. WL, whole lung. Individual relative densitometric values normalized to β-actin for each blot are presented.