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Amiloride does not inhibit Cl− uptake. Freshly isolated TI cells were incubated with amiloride alone or amiloride and CFTRinh172. Cl− uptake was then measured at 5 min. There was no significant difference in Cl− uptake among control cells (−amiloride), cells treated with amiloride (+amiloride), and cells treated with both amiloride and CFTRinh172 (n = 3 cell isolations). Results are expressed as %change from control in pmol Cl−/μg protein (%control) ± SE.
