Fig. 8.
Cl− uptake is significantly inhibited in the presence of multiple Cl− inhibitors. 36Cl uptake was measured in freshly isolated TI cells in the presence of a combination of Cl− channel inhibitors, CFTRinh172 (10 μM), NPPB (10−4 M), and DIDS (10−4 M). Results are expressed as %change from control in pmol Cl−/μg protein (%control) ± SE. A: under unstimulated conditions, there was no significant inhibition of Cl− uptake at 5 min in cells exposed to multiple inhibitors (−terbutaline, +inhibitors) compared with controls (−terbutaline, −inhibitors) (n = 3 cell isolations). B: under terbutaline-stimulated conditions, there was significant inhibition of Cl− uptake, 68%, in TI cells treated with multiple Cl− channel antagonists (+terbutaline, +inhibitors) compared with controls (+terbutaline, −inhibitors) at 5 min (n = 3, *P < 0.02).