Fig. 6.

Fibroblast proliferation by the CM is not influenced by antibodies to PDGF receptors (PDGFR). The role of PDGF-AA was assessed in the proliferative response. Quiescent NHLF were incubated with anti-PDGFR-α (40 μg/ml) and anti-PDGFR-β (40 μg/ml) 1 h before the addition of SF PDGF-BB (20 ng/ml), BMSC-CM, or CBMSC-CM. BrdU incorporation was measured by ELISA. Data represent the means ± SE from triplicate wells for each treatment group from 3 separate experiments. *Significantly different from PDGF-BB, P < 0.05. #Significantly different from SF, P < 0.05.