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Ectopic expression of epitope-tagged A46R and A52R in mammalian cells. 293 cells were transfected with equal amounts of DNA (12 μg) comprising empty vector (lanes 1 and 8); 3, 6, or 12 μg of A46R-Flag (lanes 2–4 and 9–11); or 3, 6, or 12 μg A52R-Flag (lanes 5–7 and 12–14). Proteins were then detected by immunoblotting either 24 h (lanes 1–7) or 48 h (lanes 8–14) later by using an anti-Flag antibody. The relevant molecular mass markers (in kDa) are shown on the right.
