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Effect of A46R and A52R on IL-1 signaling. 293 cells were transfected with 600 ng of empty vector (EV; black bars), 600 ng of vector encoding A46R (white bars), or 150 ng of vector encoding A52R (gray bars) for 48 h. At 6 h before harvesting, cells were stimulated with 100 ng/ml IL-1α or TNFα. NFκB reporter gene activity was then measured.
