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. 2009 Nov 25;4(11):e8035. doi: 10.1371/journal.pone.0008035

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Tiede et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) 1000 mammary epithelial cells of the indicated population were transplanted into mammary fat pads cleared of their endogenous epithelium and subsequent BLI intensity was quantified in individual mice over 12 weeks, normalized to the time of injection. (B) Representative BLI images (left panel) and alum carmine staining of mammary glands (right panel) 12 weeks after transplantation of various mammary epithelial cell populations into cleared fat pads. Luciferase activity was detected solely in mice receiving CD24⁺CD29^hi MaSCs (P4) and not in mice receiving CD24⁺CD29^lo (P5) and CD24⁻ P6 cells (middle and lower panel). Mice with strong luciferase activity showed mammary gland reconstitution, based on alum carmine staining (arrow).