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. Author manuscript; available in PMC: 2009 Nov 16.
Published in final edited form as: Stem Cells. 2008 Jan 10;26(3):756–766. doi: 10.1634/stemcells.2007-0869

Figure 3.

Figure 3

Methylation status of the SNURF/SNURPN IC involved in the regulation of paternal imprints in the Prader-Willi syndrome region in rPESC lines. (A): Southern blot analysis. The larger (upper) band corresponds to a 5.7-kilobase (kb) uncut Methyl fragment, and the smaller (lower) band corresponds to the 4.66-kb digested Unmethyl fragment. (B): Bisulfite sequencing demonstrating that oocyte-derived rPESC lines were heavily Methyl, as expected, whereas all sequenced clones of mature sperm were completely Unmethyl. In biparental ORMES-22 and muscle DNA, both Unmethyl and Methyl clones were observed. Abbreviations: Methyl, methylated; ORMES, Oregon Rhesus Monkey Embryonic Stem; rPESC, rhesus parthenogenetic embryonic stem cell; Unmethyl, unmethylated.