Table 2.
Oligonucleotides used in this study.
| Name | Sequence |
|---|---|
| For allelic replacement: | |
| GBattl | GGGGACAAGTTTGTACAAAAAAGCAGGCTGCCGCGAGCTTGGGAGGGGCTCACGACC |
| GBatt2 | GGGGACCACTTTGTACAAGAAAGCTGGGTGTTAATATGATTAAGGACGCGCTATCAAAC |
| For EMSAs: | |
| P2 | TACATTTAACAGTTAAGTATTTATTTCCTACAGTTAGGCAATATAATG |
| P2 Comp | CATTATATTGCCTAACTGTAGGAAATAAATACTTAACTGTTAAATGTA |
| P2 Mut | TACATTTAATAGTTAAGTATTTATTTCCTACAGTTAGGCAATATAATG |
| P2 MutComp | CATTATATTGCCTAACTGTAGGAAATAAATACTTAACTATTAAATGTA |
| For qRT-PCR: | |
| psmα | |
| Pl | TATCAAAAGCTTAATCGAACAATTC |
| P2 | CCCCTTCAAATAAGATGTTCATATC |
| Probe | AAAGACCTCCTTTGTTTGTTATGAAATCTTATTTACCAG |
| RNAIII | |
| Pl | GTGATGGAAAATAGTTGATGAGTTGTTT |
| P2 | GAATTTGTTCACTGTGTCGATAATCC |
| Probe | TGCACAAGATATCATTTCAACAATCAGTGACTTAGTAAAA |
| clfB | |
| Pl | TTCCAATGCGCAAGGAACTAG |
| P2 | CAGCATTTACTACAGGTTCAGCAACT |
| Probe | AGACTACGTACAGCTCTCGTTCTAACACTT |
| sdrD | |
| Pl | TCAGATGAGCAAGCTTCACCAA |
| P2 | TTGGTTGAGCATTTACCACTGATT |
| Probe | ATTCTCTTGCAAATCAGGTTGTAACGCTTCTTG |
| hla | |
| Pl | AAAAAACTGCTAGTTATTAGAACGAAAGG |
| P2 | GGCCAGGCTAAACCACTTTTG |
| Probe | CCTTCTTCGCTATAAACTCTATATTGACCAGCAAT |
| spa | |
| Pl | CAGCAAACCATGCAGATGCTA |
| P2 | GCTAATGATAATCCACCAAATACAGTTG |
| Probe | CATTACCAGAAACTGGTGAAGAAAATCCATTCATTG |
| For agr sequencing: | |
| 2803-3859F | GAATCCGCAGATATTTTGACTGTA |
| 2803-3859R | CCTCACTGTCATTATACGATTTAGT |
| 3653-4609F | CACATCTCTGTGATCTAGTTAT |
| 3653-4609R | TCCACCTACTATCACACTCT |
| 4351-5315F | AAAGAGCCATTTGCCCAATT |
| 4351-5315R | GAACTTGCGCATTTCGTTGT |
| 5108-6146F | TTCACAAATAAACTCGGATG |
| 5108-6146R | CTTACGAATTTCACTGCCTA |
| 5924-6927F | TTTCATTTGCGAAGACGATC |
| 5924-6927R | GATTCACGGAGTAGGAAATT |
| 6701-7710F | CAAATGCACTGTATAGCTGGCTT |
| 6701-7710R | GAATGAAGCAAACACTGCGT |