Fig 2. Inhibition of μ-calpain suppresses Ca2+-dependent cell death in MLIV fibroblasts.
Cells were pretreated with 1 μM of calpain II (μ-calpain) inhibitor (Calbiochem) for 15 min before experiments and apoptosis was induced as in 18. Note that apoptosis rates of pretreated cells are significantly lower than those of untreated cells. The results represent 3 separate experiments; more than 20 cells were analyzed in each experiment under each condition.