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. Author manuscript; available in PMC: 2009 Nov 16.
Published in final edited form as: Mucosal Immunol. 2008 Jan;1(1):49–58. doi: 10.1038/mi.2007.5

Figure 5.

Figure 5

Frequency and functionality of HIV-specific T cells in blood, GI tract, and BAL. (a) HIV-specific CD8 T-cell responses in blood and gut. (b) HIV-specific CD4 T-cell responses in blood and gut. (c) HIV-specific CD8 T-cell responses in blood and BAL. (d) HIV-specific CD4 T-cell responses in blood and BAL. (e) Functionality of HIV-specific CD8 and (f) CD4 T cells. Lymphocytes were stimulated with overlapping HIV peptides and stained as described in Methods. The responses are shown as the percentage of memory T cells that are HIV specific. Circles or squares denote individuals’ responses, with some individuals responding to multiple peptide pools as denoted with multiple like-colored symbols. In addition, tetramer analysis was performed on several individuals, and the frequencies of memory CD8 T cells that bind tetramer are denoted by triangles. Points depicting values for the same individual are linked by a line. P-values represent the results of Wilcoxon’s matched pairs test. The frequency of the total HIV-specific responses producing one, two, or three cytokines was determined using SPICE as described in Methods. BAL, bronchoalveolar lavage; GI, gastrointestinal.