Skip to main content
. Author manuscript; available in PMC: 2009 Nov 16.
Published in final edited form as: Am J Physiol Cell Physiol. 2004 Dec 15;288(4):C831–C839. doi: 10.1152/ajpcell.00439.2004

Fig. 7.

Fig. 7

A: relative contributions of lymphocyte function-associated antigen (LFA-1) and Mac-1 adhesion in PMN-mediated melanoma cell migration. The third and fourth bars from the top demonstrate the dramatic contribution of Mac-1 to PMN-facilitated migration. The fifth and sixth bars show that LFA-1 contributes, but to a lesser degree. As a control, the last bar shows migration with isotype-treated PMNs (P values with respect to C8161+PMN case; 55.5 s−1, viscosity = 0.7 cP). B: relative influence of adhesion between melanoma cell and PMN vs. PMN and ICAM-1 and E-selectin (EI) monolayer in PMN-facilitated melanoma extravasation. The second bar shows the case of disrupting melanoma and PMN aggregation by blocking ICAM-1 on C8161 cells that significantly reduces migration. The third and fourth bars show the case of disrupting PMN adhesion to the EI monolayer by blocking E-selectin or ICAM-1 on EI cells, which also significantly reduces migration. Both components appear to contribute equally to PMN-facilitated C8161 migration (P values with respect to C8161+IL-8/PMN case; 55.5 s−1, viscosity = 0.7 cP). All error bars are means ± SE for n ≥ 3.