Figure 10.

Significant inhibition of LPS induced PGE₂ and NO production in RAW264.7 cells after treatment with chemically synthesized Bauer Alkylamide 11. The black bars represent PGE₂ levels and the white bars represent NO levels after induction with 1 μg/ml LPS and treatment with alkylamide (n = 3). All treatments + LPS were compared to media + DMSO + LPS control that was set at 100 % PGE₂ production (4.7 ng/ml) and NO production (11.6 ng/ml). Treatments were also performed without LPS induction showing no significant differences in PGE₂ or NO production. ND indicates analysis not determined. Quercetin was used as a positive control for both studies and showed significant inhibition of PGE₂ and NO production at 10 μM (p<0.0001). * and ** are representative of p<0.05 and p<0.001. Each bar represents % of control ± standard error. Parallel cytotoxicity screens were conducted yielding no significant cytotoxicity with Bauer Alkylamide 11 at the concentrations screened (data not shown).