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. Author manuscript; available in PMC: 2010 Apr 7.
Published in final edited form as: Cancer Cell. 2009 Apr 7;15(4):283–293. doi: 10.1016/j.ccr.2009.02.015

Figure 2. Stat3 Modulates Nuclear NF-κB Retention in Tumor Cells.

Figure 2

(A) Cellular localization of p-Stat3 (green) and p-RelA (red) was observed by confocal microscopy after immunofluorescent staining of A2058 cancer cells treated with Stat3 or control siRNAs. Photos with higher magnification were shown in the upper panels. Scale = 20μm. (B) Stat3-dependent p-RelA accumulation in tumor cells. Left: Western blot analyses of nuclear and cytoplasmic p-RelA in indicated cancer cells transfected with either control or Stat3 siRNAs. HDAC1 and tubulin proteins were used as nuclear and cytoplasmic controls, respectively. Middle panel: Physical interaction of p-RelA with p-Stat3 in tumors. Nuclear extracts from A2058 tumor cells were immunoprecipitated using anti-RelA antibody or anti-Stat3 or pre-immune serum (PIS) control, followed by immunoblotting analysis with specified antibodies. Right panel: Effects of knocking down Stat3 and RelA on p-RelA vs. total RelA in the nucleus.