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. Author manuscript; available in PMC: 2009 Nov 16.
Published in final edited form as: Int J Cancer. 2008 Jul 1;123(1):14–21. doi: 10.1002/ijc.23608

Figure 2.

Figure 2

A. Representative TSP1 MSP assay results for cell lines. U, PCR product from the MSP assay using primers specific for the unmethylated allele; M, PCR product from the MSP assay using primers specific for the methylated allele. B. Representative results from the TSP1 MSP analysis of normal colon mucosa, adenomas and adenocarcinomas using primers for methylated (M) and unmethylated (U) alleles of TSP1. No methylated TSP1 was detected in any of the normal colon mucosa samples. Approximately 20% of colon neoplasms were found to have methylated TSP1. The unmethylated allele present in the tumors that were shown to have methylated TSP1 is presumably from intermixed normal tissue.