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Re-expression of TSP1 in SW-48 cells after treatment with 5′-Aza-2′deoxycytidine (5′-Aza). A. TSP1 MSP results from cells that were treated for 24, 48 and 96 hours with 5′-Aza. The presence of unmethylated DNA can be seen after 48 and 96 hours of treatment. B. TSP1 mRNA levels assessed by quantitative RT-PCR for TSP1 expression, treatment with 5′-Aza resulted in a significant increase in the levels of TSP1 mRNA in SW-48 cells.
