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. Author manuscript; available in PMC: 2009 Nov 16.
Published in final edited form as: Int J Cancer. 2008 Jul 1;123(1):14–21. doi: 10.1002/ijc.23608

Figure 6.

Figure 6

A. Assessment of TSP1 mRNA levels by quantitative RT-PCR after transfection with siRNA against TSP1. TSP1 expression is reduced to approximately 15% of basal levels by siRNA. No effect of the control siRNA on TSP1 expression is observed. B. Assessment of total and activated TGF-βin FET cells treated with TGF-β:LAP. Activated TGF-β1 is detected in FET cells that were treated with the TGF-β:LAP complex, such activation represents ~50% of the total TGF-β present in the media. TGF-β1 activation was not detected in cells in which the TGF-β:LAP complex was not added. C. Assessment of TGF-β:LAP complex activation in FET cells after treatment with siRNA against TSP1. A substantial reduction in activated TGF-β is observed. No effect on TGF-β activation is observed in the FET cells treated with the control siRNA. These experiments were done in duplicate with a representative set of results shown.