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. Author manuscript; available in PMC: 2009 Nov 16.
Published in final edited form as: Int J Cancer. 2008 Jul 1;123(1):14–21. doi: 10.1002/ijc.23608

Figure 7.

Figure 7

TSP1 activates TGF-β present in the TGF-β1:LAP complex and induces Smad signaling. A. 3TPLux luciferase reporter activity in the SW-48 cell line 48 hours after transfection with TSP1. The 3TP-Lux reporter activity is increased approximately 1.2X in the TSP1 transfected SW-48 cells and 1.8X in the SW-48 cells treated with activated TGF-β (2ng/ml) (101-B1-010, R&D Systems) when compared with non-treated SW-48 cells (*p=0.0005, Student’s t-test analysis). B. 3TPLux luciferase reporter activity in the SW-48 cell line after addition of preformed TGF-β1:LAP and transfection with TSP1. The 3TP-Lux reporter activity is increased approximately 1.3X after 96 hours of treatment compared to the baseline level. (**p= 0.003; Student’s t-test analysis).