Figure 2. Fluorescence spectra and quenching efficiencies.
(A-C) Representative composite and deconvoluted spectra showing individual PL contributions as a function of the protein-to-QD ratio for: 510 nm QD-YFP, 550 nm QD-mCherry, and 540 nm-QD-b-PE assemblies. (D-E) Plots of the corresponding quenching efficiencies along with the acceptor-sensitized emission. In panel (E), the energy transfer efficiency extracted from changes in the QD excited state lifetime are also shown for the 550 nm QD-mCherry pair. Data are corrected for the direct excitation of the b-PE. QD emission profiles were fit with a Gaussian function.