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. Author manuscript; available in PMC: 2010 Oct 5.
Published in final edited form as: J Phys Chem C Nanomater Interfaces. 2009 Oct 5;113(43):18552–18561. doi: 10.1021/jp9060329

Table I. Selected characteristics of the quantum dot - fluorescent protein pairs used in this study.

Quantum Dot Donor Fluorescent Protein Acceptor
QD λmax
emission
Quantum
yield
Conjugation
strategy
Protein
acceptor
λmax
absorption
λmax
emission
Extinction
coeff. (M-1cm-1)
Quantum
yield
R0 r
510 nm 12 % Metal-affinity YFP 516 nm 529 nm 20,200 60 % 39Å 62 Å
550 nm 20 % Metal-affinity mCherry 587 nm 610 nm 71,000 22 % 49Å 56 Å
520 nm 20 % Biotin/Streptavidin b-PE 545,565 nm 575 nm 2,410,000 98 % 44Å* ---
540 nm 19 % Biotin/Streptavidin b-PE 545,565 nm 575 nm 2,410,000 98 % 53Å* ---

YFP - yellow fluorescent protein, b-PE - b-phycoerythrin

*

R0 calculated for interactions with a single bilin chromophore Standard deviations associated with the experimental r values were ~ 5-7%.