Table I. Selected characteristics of the quantum dot - fluorescent protein pairs used in this study.
Quantum Dot Donor | Fluorescent Protein Acceptor | ||||||||
---|---|---|---|---|---|---|---|---|---|
QD λmax emission |
Quantum yield |
Conjugation strategy |
Protein acceptor |
λmax absorption |
λmax emission |
Extinction coeff. (M-1cm-1) |
Quantum yield |
R0 | r |
510 nm | 12 % | Metal-affinity | YFP | 516 nm | 529 nm | 20,200 | 60 % | 39Å | 62 Å |
550 nm | 20 % | Metal-affinity | mCherry | 587 nm | 610 nm | 71,000 | 22 % | 49Å | 56 Å |
520 nm | 20 % | Biotin/Streptavidin | b-PE | 545,565 nm | 575 nm | 2,410,000 | 98 % | 44Å* | --- |
540 nm | 19 % | Biotin/Streptavidin | b-PE | 545,565 nm | 575 nm | 2,410,000 | 98 % | 53Å* | --- |
YFP - yellow fluorescent protein, b-PE - b-phycoerythrin
R0 calculated for interactions with a single bilin chromophore Standard deviations associated with the experimental r values were ~ 5-7%.