Table II. Donor-acceptor excited state lifetimes for 550 nmDHLA QDs assembled with increasing mCherry protein. Amplitude weighted average lifetimes (τAv) are given in nanoseconds.
| Ratio of mCherry/QD | QD donor1 | mCherry acceptor2 | mCherry acceptor rise time3 |
|---|---|---|---|
| 0 | 8.51 (QD only) | 1.55 (mcherry alone) | -------- |
| 0.25 | 6.98 | 4.32 | 0.80 |
| 0.5 | 6.19 | 4.10 | 0.75 |
| 0.75 | 5.51 | 3.69 | 0.77 |
| 1 | 4.74 | 3.44 | 0.74 |
| 1.5 | 3.89 | 3.44 | 0.70 |
| 2 | 3.86 | 3.41 | 0.64 |
| 3 | 3.22 | 3.00 | 0.69 |
| 4 | 3.37 | 3.02 | 0.67 |
| 6 | 3.51 | 3.30 | 0.60 |
| 8 | 3.47 | 3.06 | 0.67 |
| 10 | 3.67 | 3.12 | 0.63 |
| 15 | 3.89 | 3.13 | 0.56 |
1
Fitted with an average of 3 or 4 lifetime components.
2
Fitted with an average of 2 or 3 lifetime components.
3
Extracted from the data shown in Figure 3B. QD and mCherry lifetimes monitored at 537 nm and 620 nm, respectively. Standard deviations were < 5% for all values.