Table 1.
Validation of hMPV-induced gene expression by real-time PCRa
| Gene symbol | GeneBank No. | Fold induction |
|---|---|---|
| GRO-α | NM 001511 | 9 |
| GRO-β | M57731 | 11 |
| IL-8 | NM 000584 | >20 |
| IP-10 | NM 001565 | >20 |
| RANTES | AF043341 | >20 |
| IL-6 | NM 000600 | >2 |
| IFNβ1 | NM 002176 | >20 |
| RIG-I | AI304317 | >20 |
| COX-2 | AY151286 | >20 |
| TRAF-1 | NM 005658 | 10 |
| IKBα | AI078167 | >20 |
| IKKε | NM 014002 | 5 |
| STAT-1 | BC002704 | >20 |
| IRF-1 | NM 002198 | >20 |
| NF-κB1 | M55643 | 10 |
| RELA/P65 | NM 021975 | >20 |
a
RNA extracted from uninfected or hMPV-infected cells for 12 h was used as template for RT-PCR, as described in Methods. Fold induction represents change in level of target gene expression, after normalization to an endogenous reference (18S) sample, in hMPV-infected versus uninfected cells.