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. Author manuscript; available in PMC: 2010 May 8.
Published in final edited form as: J Chromatogr A. 2008 Dec 27;1216(19):4313–4318. doi: 10.1016/j.chroma.2008.12.056

Fig.1.

Fig.1

HPLC chromatogram of the crude extract from Hypericum perforatum L. with the chemical structure of epigallocatechin, hyperoside, isoquercitrin, miquelianin, quercitrin, avicularin and quercetin

HPLC conditions: C18 column (150×4.6mm, 5 μm, YMC-Pack), column temperature: 30°C. Mobile phase: MeOH (eluent A) and 0.08% (v/v) H3PO4 in water (eluent B). Gradient conditions: at time 0, 95% B; at time 5 min, 70% B; at time 25 min, 55% B; at time 30 min, 50% B; at time 40 min, 50% B; at time 45 min, 95% B. Flow rate: 0.8 ml/min, monitored at 254 nm. Peak 1: epigallocatechin. Peak 2 (a, b, c): hyperoside, isoquercitrin and miquelianin. Peak 3: quercitrin. Peak 4: avicularin. Peak 5: quercetin.