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. Author manuscript; available in PMC: 2010 May 8.
Published in final edited form as: J Chromatogr A. 2008 Dec 27;1216(19):4313–4318. doi: 10.1016/j.chroma.2008.12.056

Fig.3.

Fig.3

Chromatogram of the crude extract from hypericum perforatum by high-speed countercurrent chromatography (HSCCC)

Solvent system: ethyl acetate-methanol-water (10:1:10); stationary phase: upper organic phase; mobile phase: lower aqueous phase; flow rate: 2.0 ml/min; revolution speed: 800rpm; sample: 900 mg dissolved in 10ml of lower phase. HPLC conditions: the same as Fig 1. Peak 2: mixture of hyperoside, isoquercitrin and miquelianin. Peak 3: mixture of epigallocatechin and avicularin. Peak 4: quercitrin. Pumped out peak by N2: quercetin