Table 1.
Data for inhibition of precursor self-splicing by peptoids that bind to the ribozyme when displayed on a microarray.
| Sequence R1,R2,R3,R4 | IC50 (µM)a | Relative Array Binding Signal b, c |
|---|---|---|
| 4-4-3-12 | 158 ± 14 | 1.00 |
| 4-6-3-12 | 150 ± 40 | 0.35 |
| 4-3-3-12 | 157 ± 40 | 0.66 |
| 6-4-3-12 | 209 ± 90 | 0.71 |
| 3-3-2-12 | 399 ± 14 | 0.56 |
| 7-6-2-12 | 500 ± 110 | 0.42 |
| 8-3-2-12 | 633 ± 153 | 0.48 |
| 3-4-3-12 | 817 ± 76 | 0.24 |
| 3-8-2-12 | 1217 ± 301 | 0.79 |
| 1-3-2-12 | 1320 ± 814 | 0.27 |
| 3-1-2-12 | 1733 ± 681 | 0.34 |
| 3-6-3-12 | 2200 ± 1304 | 0.35 |
| 1-5-8-12d | >5,000 | 0.00 |
| Pentamidine | 200±35 | - |
a
All reactions were completed in the presence of 1 mM nucleotide concentration of bulk yeast tRNA competitor.
b
In these assays, peptoids are conjugated to alkyne-functionalized agarose microarrays via HDCRs.
c
Values are determined by dividing the signal from the compound by the signal from 4-4-3-12 at the highest loading spot from Figure 2.
d
Compound 1-5-8-12 is a negative control that did not give signal for binding to the ribozyme on the array.