Figure 6. Potentiation of Notch target gene expression in the absence of NKAP.
DN3, DP, CD4 SP and CD8 SP thymocytes from wild-type and lck-cre NKAP cKO mice were isolated by FACS sorting. mRNA was generated and examined by quantitative PCR for CD25, Hes1, Deltex1 and RIBP mRNA expression levels, and normalized relative to the sorted wild-type population with the lowest expression of each gene (=1). The data shown is from two wild-type and three lck-cre NKAP cKO mice, each from independent sorts and independent Q-PCR. Error bars reflect SEM.