Fig. 4.
Dissociated VMN neurons from offspring of DIO and DR dams fed chow or HE diet (n = 5–7 rats/group) through gestation and weaning were categorized using calcium imaging as being GE (A, B) or GI (C, D). They were then categorized as being excited (OAE) (A, C) or inhibited (OAI) (B, D) and their sensitivity to OA assessed by the percent change in [Ca2+]i oscillation AUC produced by successive exposure to 1 fM, 1 pM, and 100 pM OA. Data are expressed as means ± SE. Data points with differing letters differ from each other by P = 0.05 or less by Bonferroni post hoc test after intergroup differences were found by two-way ANOVA with repeated measures.