Table 1.
Biophysical properties of PolybHb solutions
| PolyHb (10 g/dl) | Viscosity, cp | COP, mmHg | P50, mmHg | n | MetHb Level, % | Yield, % |
|---|---|---|---|---|---|---|
| <500 kDa | ||||||
| 20:1 | 1.4 | 50 | 34.6 | 1.2 | 2.5 | 48 |
| 30:1 | 1.4 | 48 | 28.6 | 1.0 | 2.0 | 29 |
| 40:1* | 1.0 | 18 | 27.7 | 0.8 | 3.6 | 4 |
| 50:1† | 1.0 | 18 | 23 | 0.8 | 2.8 | 3 |
| >500 kDa | ||||||
| 20:1 | 1.7 | 35 | 30.5 | 1.2 | 1.3 | 48 |
| 30:1 | 1.8 | 28 | 33.8 | 1.0 | 1.9 | 48 |
| 40:1 | 7.2 | 5 | 38.1 | 0.9 | 2.1 | 49 |
| 50:1 | 11.4 | 1 | 34.9 | 0.8 | 2.0 | 65 |
Values are biophysical properties of polymerized bovine hemoglobin (PolybHb) solutions, with concentrations adjusted as close as possible to 10g/dl, at low (<500 kDa) and high molecular mass (>500 kDa) and different cross-link densities (20:1, 30:1, 40:1, and 50:1). Viscosity was measured at a shear rate of 150 s−1; colloid osmotic pressure (COP) was measured at 25°C. P50, Po2 at which Hb/PolybHb is half-saturated with O2; n, cooperativity coefficient; MetHb, methemoglobin.
*
PolybHb (40:1)low concentration at 5.6 g/dl.
†
PolybHb (50:1)low concentration at 4.2 g/dl.