Table 1.
Primer sequences used for quantitative real-time PCR
| PCR Primer Sequence 5′ → 3′ | Melt Curve Temperature | |
|---|---|---|
| Follistatin | F: AAGACCGAACTGAGCAAGGA | 84°C |
| R: TTTTTCCCAGGTCCACAGTA | ||
| FOXO3A | F: GAACGTGGGAACTTCACTGGTGCTA | 84°C |
| R: GGTCTGCTTTGCCCACTTCCCCTT | ||
| MAFbx | F: CATCCTTATGTACACTGGTCCAAAGA | 82°C |
| R: TCCGATGATACACCCACATGTTAATG | ||
| MRF4 | F: CCCCTTCAGCTACAGACCCAAACAAGAA | 79°C |
| R: CCCCCTGGAATGATCGGAAACAC | ||
| MURF-1 | F: CTCAGTGTCCATGTCTGGAGGCCGTT | 79°C |
| R: GGCCGACTGGAGCACTCCTGTTTGTA | ||
| Myf5 | F: ATGGACGTGATGGATGGCTGCCAGTT | 83°C |
| R: GCGGCACAAACTCGTCCCCAAATT | ||
| MyoD | F: CACTACAGCGGCGACTCC | 79°C |
| R: TAGGCGCCTTCGTAGCAG | ||
| Myogenin | F: GGTGCCCAGCGAATGC | 84°C |
| R: TGATGCTGTCCACGATGGA | ||
| Myostatin | F: CTGTAACCTTCCCAGGACCA | 82°C |
| R: CCCATCCAAAAGCTCAAAA | ||
| GAPDH | F: AGCCACATCGCTCAGACA | 79°C |
| R: GCCCAATACGACCAAATCC |
F, forward; R, reverse; FOXO3A, forkhead box 3A; MAFbx, muscle atrophy F-box; MuRF-1, muscle ring finger-1; MyoD, myogenic differentiation factor; Myf5, myogenic factor 5; MRF4, muscle regulatory factor 4.