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. Author manuscript; available in PMC: 2009 Nov 16.
Published in final edited form as: Stem Cells Dev. 2006 Jun;15(3):407–421. doi: 10.1089/scd.2006.15.407

Fig. 3. Factors expressed at higher levels in neurogenesis-inhibiting astrocytes and non-neurogenic regions inhibit NSPC neuronal differentiation promoted by NBH astrocytes.

Fig. 3

(a, b, c) GFP-expressing NSPCs (green) differentiate into TuJI+ neurons (red) when co-cultured with NBH astrocytes for 4 days, in the absence (a,b) or presence of 50ng/ml IGFBP6 (c). (d) Quantification of immunofluorescent staining indicated that IGFBP6, decorin (Dcn) and enkephalin (Enk) significantly reduced neuronal differentiation (represented by the percentage of TuJI+ neurons) of NSPCs that were co-cultured with NBH astrocyte; (e) Dose-dependent inhibition of NSPC neuronal differentiation by IGFBP-6. *, p<0.05; **, p<0.01, ***, p<0.001 (unpaired, 2-tailed student's t-test).