Fig. 1.
Distribution of green fluorescent protein (GFP) in the bacteria artificial chromosome (BAC)-npy mouse strain. Phase contrast (A) and fluorescent (B) photomicrographs of a coronal section of mouse brain including cortex (ctx) and striatum (str). Note the sparse distribution of GFP-expressing neurons in striatum compared with cortex. Images shown in A and B show the same field of view. Scale bar, 250 μm. Immunohistochemical detection of choline acetyltransferase (ChAT, C), parvalbumin (parv, D) and neuropeptide Y (NPY; npy, E and F) in confocal z-stack projections of striatum. Antigen detection visualized with species selective 2°antibody conjugated to Alexa Fluor 555 (in red). Eg and Er images correspond to fluorescence detection on green and red channels, respectively. Em image is merged image of these 2 channels. F: NPY immunoreactivity (in red) in the BAC-drd2 mouse strain showing the lack of expression overlap. Insets: 1.5× zoom for more detail. Scale bars in C–F, 25 μm.