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Expression of cardiac-specific markers by late passage (P3) spongiosa hMSCs cultured in 3-D culture with differentiation cocktail. FACS analyses of differentiated spongiosa hMSCs stained by antibodies against cardiac myosin heavy chain (MYH7B), cardiac troponin I (TnI), Nkx2.5 and smooth muscle actin (SMA). The slight expression of MYH7B, TnI and Nkx2.5 by all cells and SMA expression by some single cells was revealed.
