Figure 1.

STLV-3d(Cmo8699AB) genomic organization (a) and schematic representation of PCR-based genomic walking strategy (b). (a) Non-coding long terminal repeats (LTR), coding regions for all major proteins (gag, group specific antigen; pro, protease; pol, polymerase; env, envelope; rex, regulator of expression; tax, transactivator). (b) Short tax and LTR sequences (fragments A, G, H, and I) were amplified using generic primers as previously described [7,27,31]. Using a previously described PCR-based genomic walking strategy [14], the complete proviral sequence (8913-bp) was then obtained by using STLV-3d-specific primers located within each major gene region in combination with generic PTLV primers (fragments B - F). Amplicon sizes are approximated with the solid bars. The positions of predicted donor (sd) and acceptor (sa) splice sites are shown in parentheses.