Table 1.

Summary of recovery of pyrosequencing tags for Framvaren (FV) and Cariaco (CAR) samples, along with accompanying metadata.

	Sample number-sample name
	1-FV1	2-FV2	3-FV3	4-FV4	5-CAR1	6-CAR2	7-CAR3	8-CAR4
N 454-reads total	38735	34171	24217	33962	35267	30277	28305	26714
Total eukaryotic tags > 100 bp	38280	32026	16256	32795	32876	22503	24266	23591
Unique eukaryotic tags	4280	5283	3765	5141	5983	5701	4325	4016
Total protistan tags (incl. Fungi)	23722	29402	12864	26543	30161	14453	7166	5969
Unique protistan tags (incl. Fungi)	3220	4825	3204	4439	5597	4616	2152	2070
Total and (unique) unassignable tags at 85%	1338	1153	1178	9189	2255	1724	1768	1042
	(276)	(468)	(427)	(758)	(620)	(580)	(556)	(365)
Total and (Unique) Archaeal tags	0 (0)	2 (2)	2(1)	2 (2)	0 (0)	4 (3)	6 (5)	2 (2)
Total and (Unique) Bacterial tags	0 (0)	0 (0)	1 (1)	0 (0)	0 (0)	0 (0)	0 (0)	0 (0)
Latitude/Longitude	58°09'N	58°09'N	58°11'N	58°09'N	10°30'N	10°30'N	10°40'N	10°30'N
	06°45'E	06°45'E	06°45'E	06°45'E	64°40'W	64°40'W	65°35'W	64°40'W
Temperature °C	10.7	8.4	8.1	5.8	17.9	17.7	17.6	17.6
Depth (m)	20	36	36	36	250	300	320	300
Salinity [80]	27	28	27.5	25.5	36.4	36.4	36.4	36.4
Nitrate (μmol/l)	---	---	---	---	5.22	nd	nd	0.02
Silicate (μmol/l)	---	---	---	100	31	39	41	43
Ammonium (μmol/l)	0.22	2.2	---	2.2	0.12	1.27	2.4	3.2
O2 (μmol/l)	nd	nd	nd	nd	nd	nd	nd	nd
H2S (μmol/l)	nd	668	362	600	nd	1.49	3.74	4.28
Bacteria (× 106 cells/ml)	4.6	0.78	0.43	0.61	0.487	0.149	0.18	0.244
Bact Production (H3-Leu, mg/m2/d)	670	160	----	----	347	353	1305	61
Chlorophyll a (μg/l)	1.22	nd	nd	nd	nd	nd	nd	nd
DNA conc (ng/μl)	160	170	170	120	5.58	4.55	9.12	10.34
Water volume sampled (/)	15	20	20	20	7	7	7	5
Sampling date	Sept-2005	Sept-2005	Sept-2005	May-2004	Jan-2005	Jan-2005	Jan-2005	May-2005

Nd = not detectable.

--- = not available