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. 2009 Aug 15;20(16):3660–3670. doi: 10.1091/mbc.E09-01-0009

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© 2009 by The American Society for Cell Biology

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Figure 3. — CDK5RAP2 contains the basic and Ser-rich motif. (A) Alignment of CDK5RAP2(926-956) with several known basic and Ser-rich sequences. Asterisks denote residues conserved in the motif. (B) HEK293T was double transfected with FLAG-tagged CDK5RAP2 and EB1-GFP for anti-FLAG immunoprecipitation. The immunoprecipitates (50%) and the cell extracts (5%) were analyzed on immunoblots. The CDK5RAP2 constructs used were the full-length (CDK5RAP2FL) and the 926-1208 fragment. WT, wild type; 938/939A, L938A/P939A mutant. (C) Sequence alignment of CDK5RAP2 from different mammalian species. GenBank accession numbers are as follows: chimpanzee, NP_001035901; bovine, XP_584826; dog, XP_855524; rat, XP_575844; and mouse, NP_666102. (D) HEK293T extracts coexpressing EB1 and CDK5RAP2 were subjected to anti-GFP immunoprecipitation. The immunoprecipitates (100%) and the cell extracts (5%) were analyzed. CDK5RAP2 constructs are the human protein (FLAG-tagged) and the mouse counterpart (Myc-tagged).