Figure 4.

CDK5RAP2 targets to MT distal ends in a manner dependant on EB1 interaction. (A) Stable U2OS lines were analyzed on immunoblots (IBs). Parental, parental U2OS; W1, a subline expressing GFP-CDK5RAP2; M3, a subline expressing the L938A/P939A mutant (GFP-938/939A). (B) The stable lines were immunostained for MTs (anti-α-tubulin). (C) Time-lapse microscopy was performed on the stable cells. mCherry-α-tubulin was transiently expressed at low levels. Time series of the boxed areas are enlarged. Bars, 10 μm.