Figure 5.

Dilution potential measurements for Na⁺ and Cl⁻. (A) Permeability for Na⁺ and Cl⁻. Dilution potential measurements revealed no difference in permeability for Na⁺ (white bars) and Cl⁻ (dark gray bars) for vector controls and TRa-4 cells (n = 6, 6), whereas TRa-8 cells showed reduced permeabilities for both ions (P_Na: *** p < 0.001; P_Cl: *** p < 0.001; n = 5). (B) Changes in permeability for P_Na and P_Cl in TRa-8 cells did not affect the P_Na/P_Cl ratio, demonstrating no altered charge preference after TRIC-a overexpression (n = 6, 6, and 5, respectively).