Table 1.
Pck is critical for glucose fermentation to succinate by derivatives of KJ073
| Strain* | Genetic modification | Glucose consumed (mM) | Succinate (mM) | Yield† (mol/mol) | Acetate (mM) | Pyruvate (mM) |
|---|---|---|---|---|---|---|
| KJ073 | None | 301 ± 0 | 364 ± 25 | 1.21 ± 0.08 | 113 ± 8 | 3 ± 1 |
| XZ320 | KJ073, Δ ppc | 278 ± 0 | 348 ± 12 | 1.25 ± 0.04 | 100 ± 5 | — |
| XZ341 | KJ073, Δ maeA | 278 ± 0 | 339 ± 8 | 1.22 ± 0.03 | 106 ± 13 | — |
| XZ396 | KJ073, Δ maeB | 278 ± 0 | 337 ± 22 | 1.22 ± 0.08 | 116 ± 8 | — |
| XZ332 | KJ073, Δ pck | 92 ± 21 | 42 ± 11 | 0.46 ± 0.11 | 28 ± 5 | 32 ± 4 |
| XZ332 (pLOI4677) | Δ pck, pck overexpression | 273 ± 0 | 260 ± 36 | 0.95 ± 0.13 | 78 ± 4 | 32 ± 5 |
*All fermentations were performed in fleakers containing NBS medium with 5% glucose and 100 mM potassium bicarbonate.
†Yield was calculated as mol succinate produced per mol glucose metabolized.