Table 1. Calibration results for the 488-nm and the 633-nm lines using Rhodamine green and Cy5 dyes by fluorescence correlation spectroscopy.
Dye | Laser line (nm) | τdiff § (µsec) | z § (µm) | ω§ (µm) | S§ | V eff § (fL) |
Rhodamine Green | 488 | 22 | 1.162 | 0.157 | 7.4 | 0.159 |
Cy5 | 633 | 38 | 2.124 | 0.219 | 9.7 | 0.567 |
τdiff, z, ω, S, and V eff are defined in Equations (1), (2), and (3) (see text).