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. 2009 Nov 26;4(11):e8074. doi: 10.1371/journal.pone.0008074

Table 1. Calibration results for the 488-nm and the 633-nm lines using Rhodamine green and Cy5 dyes by fluorescence correlation spectroscopy.

Dye Laser line (nm) τdiff § (µsec) z § (µm) ω§ (µm) S§ V eff § (fL)
Rhodamine Green 488 22 1.162 0.157 7.4 0.159
Cy5 633 38 2.124 0.219 9.7 0.567
§

τdiff, z, ω, S, and V eff are defined in Equations (1), (2), and (3) (see text).