Table 2. Results for the measurement of dye-labeled gene probes by fluorescence autocorrelation spectroscopy.
Dye-Labeled Gene Probe | Laser line (nm) | τdiff § (µsec) | Nac § | Nc (kHz) | Concentration (nM) |
Rhodamine Green-labeled probe | 488 | 47 | 3.952 | 10.787 | 41.29 |
Cy5-labeled probe | 633 | 97 | 0.178 | 1.739 | 0.53 |
τdiff and Nac are defined in Equations (1) and (2) (see text).