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. 2009 Nov 25;4(11):e8031. doi: 10.1371/journal.pone.0008031

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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A. Constructs used to study the effects of having three acceptors in a FRET complex. Blue “cans” depict the Cerulean donor, Yellow depicts Venus acceptors, and gray depicts Amber. B. Table showing the FRET efficiencies of VCAA, ACVA, ACAV and VCVV measured by E-FRET, as well as the measured acceptor to donor ratio (A/D) for each construct. C. Energy transfer rates and their propagated error were calculated from the measured Cerulean lifetime of ACA and the FRET efficiencies measured by E-FRET. The Sum column is the arithmetic sum of the VCAA, ACVA and ACAV transfer rates with propagated error, and by theory should equal the transfer rate measured for VCVV.