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. 2009 Nov 25;4(11):e8031. doi: 10.1371/journal.pone.0008031

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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E-FRET was used to measure the FRET efficiencies and the acceptor intensity (Venus) for each cell expressing members of the VCV series (A; VCV, ACV, and VCA) or the VCVV series (B; VCVV, ACVA, VCAA, ACAV), and these values are plotted as a function of the exposure normalized Venus intensity. Dashed lines indicates linear regression fitting for each data set to calculate the y-intercept as an estimate of the amount of intramolecular FRET in the absence of intermolecular FRET. Note that the data is plotted on a semi-log scale to more easily reveal the full range of the Venus intensity, but in so doing make linear plots appear curved.