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. Author manuscript; available in PMC: 2009 Nov 17.
Published in final edited form as: Immunity. 1996 Jul;5(1):63–72. doi: 10.1016/s1074-7613(00)80310-6

Figure 1. Cb10 CTLs Are Restricted by H2-M3wt.

Figure 1

(A) Competition of fMet peptides for presentation to Cb10 CTL effectors. Shown is specific lysis of EL4 target cells coated with HPLC-purified Fr40 plus the indicated concentrations of fMet (closed symbols) or nonformylated (open symbol) competitor peptides. The E:T ratio used was 5:1. Lysis of Fr40-coated targets in the absence of competitors was 47.5%.

(B) Effects of target cell MHC and β2m expression on presentation to Cb10 CTL effectors. Target cells differing in MHC haplotype and β2m expression were coated with uninoculated bacterial culture media (TSB; open symbols) or LmSN (closed symbols) and assayed for lysis by Cb10 CTLs at the indicated E:T. EL4 (H-2b) and R1.1 (H-2k) cells express β2m. R1.E is a β2m-negative mutant of R1.1.

(C) Effects of H2-M3wt expression on presentation to Cb10 CTL effectors. Shown is lysis of TSB- (open symbols) or LmSN- (closed symbols) coated B10.CAS2, TR8-4a, and control EL4 targets at the indicated E:T ratios. B10.CAS2 fibroblasts express a null H2-M3 allele (H2-M3cas2), whereas TR8-4a transfectants and EL4 express H2-M3wt.