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. Author manuscript; available in PMC: 2009 Nov 17.
Published in final edited form as: Immunity. 1996 Jul;5(1):63–72. doi: 10.1016/s1074-7613(00)80310-6

Figure 6. fMet Peptides Corresponding to the N Terminus of LemA Efficiently Target Lysis by Cb10 CTLs.

Figure 6

Stocks of synthetic peptides corresponding to the N-terminal 5, 6, 7, or 8 residues of LemA were diluted in PBS and coated onto EL4 targets at the indicated final concentrations. All peptides were synthesized with an N-formylmethionine (fM), except for LemA (1–6), which was synthesized with an unformylated methionine. Peptide-coated targets were assayed for lysis in a 4 hr CTL assay with Cb10 CTL effectors at an E:T of 5:1. Specific lysis of peptide-coated targets in the absence of CTLs was less than 5%.