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. Author manuscript; available in PMC: 2009 Nov 17.
Published in final edited form as: Eur J Cancer. 2008 Jul 7;44(12):1770–1779. doi: 10.1016/j.ejca.2008.05.016

Fig. 4. CEACAM6 suppression completely blocks invasion of MCF-7:5C breast cancer cells.

Fig. 4

(A) siRNA-mediated gene knockdown of CEACAM6 was verified by Western blot (top panel) and qRT-PCR (bottom panel). For qRT-PCR experiments, relative expression of CEACAM6 gene was calculated using the 2 delta CT method, where 18S-rRNA was used as the endogenous control gene. All reactions were performed in triplicates and the error bar represents the standard deviation.. (B) Matrigel invasion assay of siControl and siCEACAM6-transfected MCF-7:5C cells. (C) Immunoblot analysis of MCF-7:5C cells transfected with CEACAM6 siRNA or control siRNA for 72 hours. β-actin was used as a loading control. (D) Western blot analyses of E-cadherin, β-catenin, N-cadherin, Akt, and pAKT protein expression in MCF-7, MCF-7:5C, and MCF-7:2A cells.