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. Author manuscript; available in PMC: 2009 Nov 17.
Published in final edited form as: Cardiovasc Res. 2007 Mar 28;75(1):129–138. doi: 10.1016/j.cardiores.2007.03.019

Figure 4.

Figure 4

A. Stretched cardiomyocytes express markers of hypertrophy. Northern blot showing increased ANF (atrial natriuretic factor) mRNA levels in stretched cardiomyocytes. GAPDH is loading control. B. Phosphorylation of STAT 3 increases with continued stretch of cardiomyocytes (1 hr and 24 hr); overall STAT3 protein levels remain constant. C. CLP-1 is released from p-TEFb complexes in stretch-induced hypertrophic cardiomyocytes. Immunoblot of p-TEFb components in stretched and non-stretched cardiomyocytes showing markedly reduced levels of CLP-1 in P-TEFb complexes from cardiomyocytes stretched for 48 hours; cyclin T1 and cdk9 levels remain unchanged. D. Total levels of p-TEFb components (free and complexed) remain unchanged with stretch. GAPDH is loading control.